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Proteins were precipitated with 20% v/v TCA (500 μL), centrifuged (11,000 rpm, 5 min, 4 °C), and the pellet was washed three times with EtOH/EtOAc (1:1, 3 × 100 μL)

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During the Appointment A licensed registered nurse confirms the treatment order, reviews relevant information, and establishes IV access